Conservation management for a species generally rests on intraspecific units, while identification of their geographic boundaries is necessary for the implementation.Intraspecific units can be discriminated using population genetic methods, yet an analytical approach is still lacking for detecting their geographic boundaries.Here, based on landscap
In this study, we developed a novel, multiplex qPCR assay for simultaneous detection of RIG-1, MDA5, and IFIT-1 at the mRNA level.The assay was validated in A549 cells transfected with in vitro transcribed RNAs.Both exogenous RNA-GFP and self-amplifying (saRNA-GFP) induced significant expression of RIG-1, MDA5, IFIT-1, as well as type I and III int
Aging is a universal nature long process, typical of living beings.Its growth at the world population level is notorious Traditional Art and it is not exclusive to developed countries.The elderly patient presents great challenges due to the alterations caused by the aging process, which is accompanied by decreased response capacity with uneven dete
Although it is a front-line in tuberculosis treatment, rifampicin (RF) exhibits poor oral bioavailability and hepatotoxicity.Rectal mucoadhesive and in situ rectal gels were developed to overcome drug drawbacks.A RF/polyethylene glycol 6000 co-precipitate was first prepared in different ratios.Based on the drug solubility, the selected ratio was in
Fusarium wilt (FW) disease is the key constraint to grain legume production worldwide.The projected climate change is likely to exacerbate the current scenario.Of the various plant protection measures, genetic improvement of the disease resistance of crop cultivars remains the most economic, straightforward and environmental-friendly option to miti